>EcoRV 1456 3646 4281 5753 12150 15802 -1 >HindIII 1293 2899 3424 3592 3643 5187 5585 9957 -1 >EcoRI 540 2253 2507 3644 4157 4515 4750 5077 5584 11408 -1 >SacI 438 545 923 1272 1659 3013 3823 5827 10791 21462 -1