>HindIII 3751 5548 5918 5997 22173 22173 -1 >EcoRI 1963 3894 11254 11604 16344 -1 >EcoRV 1824 7394 12004 24793 -1 >SacI 872 1485 1906 2271 2325 3493 4328 10652 17137 17494 -1