>HindIII 3494 4488 8189 8505 9425 9584 -1 >EcoRI 1693 1739 2227 2882 3330 4572 7789 9374 11356 -1 >EcoRV 2061 10544 34439 34439 -1 >SacI 1224 1321 1820 2576 3700 5361 13051 16284 -1