>HindIII 1445 1648 1682 1874 1994 3814 5104 6574 8707 9530 -1 >EcoRI 1887 1919 9497 11849 15233 -1 >EcoRV 1061 2045 3045 5007 6660 6748 7768 9918 -1 >SacI 2920 12667 26294 -1