>HindIII 1738 4086 4988 10153 28780 28780 -1 >EcoRI 915 1831 2465 2668 7560 9308 11388 11668 -1 >EcoRV 1401 1833 4846 5351 5793 6392 9589 12324 -1 >SacI 1013 1179 4292 6563 8585 9147 16484 -1