RNA-Seq Coverage (Tissues) Track Settings

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RNA-Seq Coverage for Different Tissues (All RNA Seq Tracks)

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Track height:	pixels (range: 11 to 110)
Data view scaling:	Always include zero:
Vertical viewing range:	min:	max: (range: -5e+06 to 5e+06)
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Select subtracks by sex and tissue: (help)

All	*Sex*	Female	Male
*Tissue*
Head
Thorax
Abdomen
Digestive System
Virgin Female Ovary
Virgin Male Testes
Gonad
Reproductive Tract
Carcass
Whole Body

List subtracks: only selected/visible all ()
	hide Configure	Female Head	RNA-seq for Female Head	Schema
	hide Configure	Male Head	RNA-seq for Male Head	Schema
	hide Configure	Female Thorax	RNA-seq for Female Thorax Without Digestive System	Schema
	hide Configure	Male Thorax	RNA-seq for Male Thorax Without Digestive System	Schema
	hide Configure	Female Abdomen	RNA-seq for Female Abdomen Without Digestive or Reproductive System	Schema
	hide Configure	Male Abdomen	RNA-seq for Male Abdomen Without Digestive or Reproductive System	Schema
	hide Configure	Female Digestive System	RNA-seq for Female Digestive Plus Excretory System	Schema
	hide Configure	Male Digestive System	RNA-seq for Male Digestive Plus Excretory System	Schema
	hide Configure	Virgin Female Ovary	RNA-seq for Virgin Female Ovary	Schema
	hide Configure	Virgin Male Testes	RNA-seq for Virgin Male Testes	Schema
	hide Configure	Female Gonad	RNA-seq for Female Gonad	Schema
	hide Configure	Male Gonad	RNA-seq for Male Gonad	Schema
	hide Configure	Female Reproductive System	RNA-seq for Female Reproductive System Without Gonad	Schema
	hide Configure	Male Reproductive System	RNA-seq for Male Reproductive System Without Gonad	Schema
	hide Configure	Virgin Female Carcass	RNA-seq for Virgin Female Carcass	Schema
	hide Configure	Virgin Male Carcass	RNA-seq for Virgin Male Carcass	Schema
	hide Configure	Female Whole Body	RNA-seq for Female Whole Body	Schema
	hide Configure	Male Whole Body	RNA-seq for Male Whole Body	Schema

Description

These tracks show the RNA-Seq coverage for different D. ananassae tissues. The RNA-Seq read coverage was normalized by a wigsum of 100,000,000 (1 million 100bp reads). The RNA-Seq reads were mapped against the D. ananassae DanaCAF1 genome assembly using HISAT2. RNA-Seq reads from multiple replicates were combined together. The read coverage tracks were produced by the bam2wig.py program in RSeQC.

The RNA-Seq data were retrieved from the NCBI Sequence Read Archive using the BioProject accession numbers PRJNA257286 and PRJNA388952.

References

Yang H, Jaime M, Polihronakis M, Kanegawa K, Markow T, Kaneshiro K, Oliver B. Re-annotation of eight Drosophila genomes. Life Sci Alliance. 2018 Dec 24;1(6):e201800156.

Rogers RL, Shao L, Sanjak JS, Andolfatto P, Thornton KR. Revised annotations, sex-biased expression, and lineage-specific genes in the Drosophila melanogaster group. G3 (Bethesda). 2014 Oct 1;4(12):2345-51.

Kim D, Langmead B, Salzberg SL. HISAT: a fast spliced aligner with low memory requirements. Nat Methods. 2015 Apr;12(4):357-60.

Wang L, Wang S, Li W. RSeQC: quality control of RNA-seq experiments. Bioinformatics. 2012 Aug 15;28(16):2184-5.