Iso-Seq Read Alignments Track Settings
 
Minimap2 Alignments of D. grimshawi Iso-Seq Reads   (All RNA Seq Tracks)

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Display read names
Minimum alignment quality:

Color track by bases: Help on base coloring

Alignment Gap/Insertion Display Options Help on display options
Draw double horizontal lines when both genome and query have an insertion
Draw a vertical purple line for an insertion at the beginning or end of the
query, orange for insertion in the middle of the query
Draw a vertical green line where query has a polyA tail insertion

Additional coloring modes:
Color by strand (blue for +, red for -)
Use gray for
Use R,G,B colors specified in user-defined tag
No additional coloring

Display data as a density graph:

BAM configuration help

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 Adult Females (Rep. 1)  Adult Females Iso-Seq - Replicate 1 (SRR6840922)   Schema 
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 Adult Females (Rep. 2)  Adult Females Iso-Seq - Replicate 2 (SRR6840921)   Schema 
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 Adult Males (Rep. 1)  Adult Males Iso-Seq - Replicate 1 (SRR6840924)   Schema 
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 Adult Males (Rep. 2)  Adult Males Iso-Seq - Replicate 2 (SRR6840923)   Schema 

Description

This track shows the alignments of D. grimshawi Iso-Seq reads against the D. grimshawi genome assembly (DgriCAF1) using Minimap2. The Iso-Seq data were obtained from the NCBI Sequence Read Archive under the accession number SRP135764.

References

Yang H, Jaime M, Polihronakis M, et al. Re-annotation of eight Drosophila genomes. Life Sci Alliance. 2018;1(6):e201800156. Published 2018 Dec 24. doi:10.26508/lsa.201800156

Li H. Minimap2: pairwise alignment for nucleotide sequences. Bioinformatics. 2018;34(18):3094-3100. doi:10.1093/bioinformatics/bty191