Schema for LTRharvest - LTR Fragments Identified by LTRharvest
  Database: DgriCAF1    Primary Table: ltrdigest_TSD    Row Count: 359   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom scaffold_10014varchar(255) values
chromStart 104int(10) unsigned range
chromEnd 4883int(10) unsigned range
name repeat_region1_TSDvarchar(255) values
score 1000int(10) unsigned range
strand +char(1) values
thickStart 104int(10) unsigned range
thickEnd 4883int(10) unsigned range
reserved 0int(10) unsigned range
blockCount 2int(10) unsigned range
blockSizes 4,4longblob  
chromStarts 0,4775longblob  

Sample Rows
 
binchromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStarts
585scaffold_100141044883repeat_region1_TSD1000+1044883024,40,4775
585scaffold_100301083519982repeat_region2_TSD1000-1083519982024,40,9143
585scaffold_1010946979217repeat_region3_TSD1000-46979217024,40,4516
585scaffold_10363157810435repeat_region4_TSD1000-157810435024,40,8853
585scaffold_10594112repeat_region5_TSD1000+94112024,40,4099
585scaffold_109526021875repeat_region6_TSD1000+6021875024,40,1269
585scaffold_1139228028429repeat_region7_TSD1000-28028429024,40,5623
585scaffold_11723710708repeat_region8_TSD1000-710708024,40,10697
585scaffold_1205883267repeat_region9_TSD1000+883267024,40,3175
585scaffold_12333606047repeat_region10_TSD1000+606047024,40,5983

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

LTRharvest (ltrdigest) Track Description
 

Description

LTRharvest is used to identify putative long terminal repeats (LTR) retrotransposons in the D. grimshawi genome using the following parameters:

-seed 76 -minlenltr 116 -maxlenltr 800 -mindistltr 2280 -maxdistltr 8773 -similar 91 -xdrop 7 -mat 2 -mis -2 -ins -3 -del -3 -mintsd 4 -maxtsd 20 -vic 60 -overlaps best

Additional features within the LTR retrotransposons are annotated by LTRdigest with the following parameters:

-pptradius 30 -pptlen 8 30 -pptrprob 0.97 -uboxlen 3 30 -pptuprob 0.91 -pbsradius 30 -pbsalilen 11 30 -pbsoffset 0 5 -pbstrnaoffset 0 40 -pbsmaxedist 1 -pbsmatchscore 5 -pbsmismatchscore -10 -pbsinsertionscore -20 -pbsdeletionscore -20 -pdomevalcutoff 1e-6 -maxgaplen 50

The subset of candidates that show significant similarity to Pfam protein domains within LTR retrotransposons are selected and then clustered using the ltrclustering program with the following parameters:

-psmall 80 -plarge 30

References