Schema for D. mel Transcripts - BLAT Alignment of D. melanogaster Transcripts
  Database: DmirGB2    Primary Table: DmelTranscriptsBLAT    Row Count: 29,665   Data last updated: 2024-01-05
fieldexampleSQL type info
chrom CM001516varchar(255) values
chromStart 15218int(10) unsigned range
chromEnd 16790int(10) unsigned range
name Cyp311a1-RBvarchar(255) values
score 490int(10) unsigned range
strand +char(1) values
thickStart 15218int(10) unsigned range
thickEnd 16790int(10) unsigned range
reserved 0int(10) unsigned range
blockCount 8int(11) range
blockSizes 96,376,113,87,93,78,183,201longblob  
chromStarts 0,165,592,707,847,1000,1155...longblob  
id FBtr0073640varchar(255) values
description FBgn0030367longblob  

Sample Rows
 
chromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStartsiddescription
CM0015161521816790Cyp311a1-RB490+15218167900896,376,113,87,93,78,183,2010,165,592,707,847,1000,1155,1371FBtr0073640FBgn0030367
CM0015161721317984CG14106-RA388+17213179840375,69,1170,156,654FBtr0075858FBgn0260459
CM0015161856520911CG4281-RA516+185702091101642,51,45,10,66,51,75,69,99,51,75,195,81,81,243,570,72,184,282,324,435,566,770,923,1216,1378,1453,1702,1849,1930,2289FBtr0070339FBgn0025626
CM0015162372930122Sik2-RA620+2372929360033111,263,62,289,430,52,33,93,48,100,152,12,66,62,37,33,84,85,74,39,3,27,144,159,30,121,32,10,35,12,40,54,300,121,450,578,969,1634,1689,1908,2016,2072,2432,2698,2805,3033,3098,3135,3207,3348,3439,3624,3666,3678,3900,4182,4431,5510,5692, ...FBtr0070340FBgn0025625
CM0015163009231762CG4313-RE606-30602317300932,52,19,357,24,24,33,81,2700,57,129,510,867,1022,1079,1172,1400FBtr0340109FBgn0025632
CM0015163009231765CG4313-RC604-30602317300932,52,19,357,24,24,33,81,2730,57,129,510,867,1022,1079,1172,1400FBtr0340107FBgn0025632
CM0015163009232385CG4313-RD640-306023235701132,52,19,357,24,24,33,81,265,360,290,57,129,510,867,1022,1079,1172,1400,1734,2264FBtr0340108FBgn0025632
CM0015164066044370moody-RB660+422754435001127,15,140,523,282,49,95,61,129,69,570,247,1615,1844,2511,2796,2917,3212,3327,3522,3653FBtr0303043FBgn0025631
CM0015164066044370moody-RD660+422754425001127,15,140,523,282,49,93,61,129,69,570,247,1615,1844,2511,2796,2919,3212,3327,3522,3653FBtr0303045FBgn0025631
CM0015164155144370moody-RA656+422754435001036,140,523,282,49,95,61,129,69,570,724,953,1620,1905,2026,2321,2436,2631,2762FBtr0070341FBgn0025631

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

D. mel Transcripts (DmelTranscriptsBLAT) Track Description
 

Description

D. melanogaster transcripts annotated by FlyBase were aligned against the D. miranda genome assembly using a translated BLAT search. The thicker boxes denote the coding regions within the transcripts and the thinner boxes denote the untranslated regions.

Methods

D. melanogaster transcripts were mapped against the D. miranda genome assembly using BLAT with the following parameters: -q=rnax -t=dnax -mask=lower

The transcript alignments are analyzed by pslReps to identify the best alignments within the genome assembly that have a minimum alignment ratio of 0.25 (-minAli=0.25). The alignments are then filtered by pslCDnaFilter using the following parameters:

  -minId=0.35 -minCover=0.15 -localNearBest=0.010 -minQSize=20
  -ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap

References

Kent WJ. BLAT--the BLAST-like alignment tool. Genome Res. 2002 Apr;12(4):656-64.