Schema for LTRharvest - LTR Fragments Identified by LTRharvest
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Database: DmirGB2 Primary Table: ltrdigest_LTR Row Count: 171   Data last updated: 2022-10-20
field | example | SQL type | info |
bin | 591 | smallint(5) unsigned | range |
chrom | CM001516 | varchar(255) | values |
chromStart | 799661 | int(10) unsigned | range |
chromEnd | 811467 | int(10) unsigned | range |
name | LTR_retrotransposon1_LTR | varchar(255) | values |
score | 1000 | int(10) unsigned | range |
strand | - | char(1) | values |
thickStart | 799661 | int(10) unsigned | range |
thickEnd | 811467 | int(10) unsigned | range |
reserved | 0 | int(10) unsigned | range |
blockCount | 2 | int(10) unsigned | range |
blockSizes | 342,343 | longblob | |
chromStarts | 0,11463 | longblob | |
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Sample Rows
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bin | chrom | chromStart | chromEnd | name | score | strand | thickStart | thickEnd | reserved | blockCount | blockSizes | chromStarts |
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591 | CM001516 | 799661 | 811467 | LTR_retrotransposon1_LTR | 1000 | - | 799661 | 811467 | 0 | 2 | 342,343 | 0,11463 |
591 | CM001516 | 818052 | 823301 | LTR_retrotransposon2_LTR | 1000 | - | 818052 | 823301 | 0 | 2 | 173,174 | 0,5075 |
592 | CM001516 | 938317 | 944551 | LTR_retrotransposon3_LTR | 1000 | + | 938317 | 944551 | 0 | 2 | 100,96 | 0,6138 |
592 | CM001516 | 1027583 | 1033872 | LTR_retrotransposon4_LTR | 1000 | - | 1027583 | 1033872 | 0 | 2 | 272,276 | 0,6013 |
593 | CM001516 | 1143635 | 1152251 | LTR_retrotransposon5_LTR | 1000 | + | 1143635 | 1152251 | 0 | 2 | 266,268 | 0,8348 |
596 | CM001516 | 1559483 | 1566075 | LTR_retrotransposon6_LTR | 1000 | + | 1559483 | 1566075 | 0 | 2 | 156,154 | 0,6438 |
597 | CM001516 | 1576838 | 1581077 | LTR_retrotransposon7_LTR | 1000 | + | 1576838 | 1581077 | 0 | 2 | 294,290 | 0,3949 |
597 | CM001516 | 1584254 | 1595723 | LTR_retrotransposon8_LTR | 1000 | + | 1584254 | 1595723 | 0 | 2 | 189,199 | 0,11270 |
601 | CM001516 | 2174690 | 2180261 | LTR_retrotransposon9_LTR | 1000 | + | 2174690 | 2180261 | 0 | 2 | 283,284 | 0,5287 |
601 | CM001516 | 2204242 | 2209003 | LTR_retrotransposon10_LTR | 1000 | - | 2204242 | 2209003 | 0 | 2 | 311,312 | 0,4449 |
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Note: all start coordinates in our database are 0-based, not
1-based. See explanation
here.
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LTRharvest (ltrdigest) Track Description
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Description
LTRharvest
is used to identify putative long terminal repeats (LTR)
retrotransposons in the D. miranda genome using the following parameters:
-seed 76 -minlenltr 116 -maxlenltr 800 -mindistltr 2280 -maxdistltr 8773 -similar 91 -xdrop 7
-mat 2 -mis -2 -ins -3 -del -3 -mintsd 4 -maxtsd 20 -vic 60 -overlaps best
Additional features within the LTR retrotransposons are annotated by
LTRdigest
with the following parameters:
-pptradius 30 -pptlen 8 30 -pptrprob 0.97 -uboxlen 3 30 -pptuprob 0.91 -pbsradius 30
-pbsalilen 11 30 -pbsoffset 0 5 -pbstrnaoffset 0 40 -pbsmaxedist 1 -pbsmatchscore 5
-pbsmismatchscore -10 -pbsinsertionscore -20 -pbsdeletionscore -20 -pdomevalcutoff 1e-6
-maxgaplen 50
The subset of candidates that show significant similarity to Pfam protein domains
within LTR retrotransposons are selected and then clustered using the ltrclustering program
with the following parameters:
-psmall 80 -plarge 30
References
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Ellinghaus D, Kurtz S, and Willhoeft U.
LTRharvest,
an efficient and flexible software for de novo detection of LTR retrotransposons.
BMC Bioinformatics, 9:18, 2008.
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Steinbiss S, Willhoeft U, Gremme G, and Kurtz S.
Fine-grained
annotation and classification of de novo predicted LTR retrotransposons.
Nucleic Acids Research, 37(21):7002-7013 (2009).
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Steinbiss S, Kastens S, and Kurtz S.
LTRsift: a graphical user interface for semi-automatic classification and postprocessing of de novo detected LTR retrotransposons.
Mob DNA. 2012 Nov 7;3(1):18.
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