Schema for LTRharvest - LTR Fragments Identified by LTRharvest

Database: DmirGB2 Primary Table: ltrdigest_RR_tract Row Count: 23 Data last updated: 2022-10-20

field	example	SQL type	info
`bin`	608	`smallint(5) unsigned`	range
`chrom`	CM001516	`varchar(255)`	values
`chromStart`	3130432	`int(10) unsigned`	range
`chromEnd`	3130447	`int(10) unsigned`	range
`name`	LTR_retrotransposon14_RR_tract	`varchar(255)`	values
`score`	1000	`int(10) unsigned`	range
`strand`	-	`char(1)`	values

Sample Rows

bin	chrom	chromStart	chromEnd	name	score	strand
608	CM001516	3130432	3130447	LTR_retrotransposon14_RR_tract	1000	-
610	CM001516	3407727	3407738	LTR_retrotransposon15_RR_tract	1000	+
655	CM001516	9279271	9279280	LTR_retrotransposon20_RR_tract	1000	+
657	CM001516	9527299	9527310	LTR_retrotransposon21_RR_tract	1000	+
691	CM001516	13915942	13915950	LTR_retrotransposon26_RR_tract	1000	-
691	CM001516	13935878	13935890	LTR_retrotransposon27_RR_tract	1000	-
695	CM001516	14448974	14448988	LTR_retrotransposon29_RR_tract	1000	+
743	CM001516	20754086	20754098	LTR_retrotransposon33_RR_tract	1000	+
591	CM001517	908683	908691	LTR_retrotransposon40_RR_tract	1000	-
592	CM001517	956130	956141	LTR_retrotransposon41_RR_tract	1000	+

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

LTRharvest (ltrdigest) Track Description


	Description LTRharvest is used to identify putative long terminal repeats (LTR) retrotransposons in the D. miranda genome using the following parameters: `-seed 76 -minlenltr 116 -maxlenltr 800 -mindistltr 2280 -maxdistltr 8773 -similar 91 -xdrop 7 -mat 2 -mis -2 -ins -3 -del -3 -mintsd 4 -maxtsd 20 -vic 60 -overlaps best` Additional features within the LTR retrotransposons are annotated by LTRdigest with the following parameters: `-pptradius 30 -pptlen 8 30 -pptrprob 0.97 -uboxlen 3 30 -pptuprob 0.91 -pbsradius 30 -pbsalilen 11 30 -pbsoffset 0 5 -pbstrnaoffset 0 40 -pbsmaxedist 1 -pbsmatchscore 5 -pbsmismatchscore -10 -pbsinsertionscore -20 -pbsdeletionscore -20 -pdomevalcutoff 1e-6 -maxgaplen 50` The subset of candidates that show significant similarity to Pfam protein domains within LTR retrotransposons are selected and then clustered using the ltrclustering program with the following parameters: `-psmall 80 -plarge 30` References Ellinghaus D, Kurtz S, and Willhoeft U. LTRharvest, an efficient and flexible software for de novo detection of LTR retrotransposons. BMC Bioinformatics, 9:18, 2008. Steinbiss S, Willhoeft U, Gremme G, and Kurtz S. Fine-grained annotation and classification of de novo predicted LTR retrotransposons. Nucleic Acids Research, 37(21):7002-7013 (2009). Steinbiss S, Kastens S, and Kurtz S. LTRsift: a graphical user interface for semi-automatic classification and postprocessing of de novo detected LTR retrotransposons. Mob DNA. 2012 Nov 7;3(1):18.

Description

References