Schema for LTRharvest - LTR Fragments Identified by LTRharvest
  Database: DmirGB2    Primary Table: ltrdigest_TSD    Row Count: 171   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 591smallint(5) unsigned range
chrom CM001516varchar(255) values
chromStart 799657int(10) unsigned range
chromEnd 811471int(10) unsigned range
name repeat_region1_TSDvarchar(255) values
score 1000int(10) unsigned range
strand -char(1) values
thickStart 799657int(10) unsigned range
thickEnd 811471int(10) unsigned range
reserved 0int(10) unsigned range
blockCount 2int(10) unsigned range
blockSizes 4,4longblob  
chromStarts 0,11810longblob  

Sample Rows
 
binchromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStarts
591CM001516799657811471repeat_region1_TSD1000-799657811471024,40,11810
591CM001516818048823305repeat_region2_TSD1000-818048823305024,40,5253
592CM001516938313944555repeat_region3_TSD1000+938313944555024,40,6238
592CM00151610275791033876repeat_region4_TSD1000-10275791033876024,40,6293
593CM00151611436311152255repeat_region5_TSD1000+11436311152255024,40,8620
596CM00151615594791566079repeat_region6_TSD1000+15594791566079024,40,6596
597CM00151615768341581081repeat_region7_TSD1000+15768341581081024,40,4243
597CM00151615842491595728repeat_region8_TSD1000+15842491595728025,50,11474
601CM00151621746862180265repeat_region9_TSD1000+21746862180265024,40,5575
601CM00151622042382209007repeat_region10_TSD1000-22042382209007024,40,4765

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

LTRharvest (ltrdigest) Track Description
 

Description

LTRharvest is used to identify putative long terminal repeats (LTR) retrotransposons in the D. miranda genome using the following parameters:

-seed 76 -minlenltr 116 -maxlenltr 800 -mindistltr 2280 -maxdistltr 8773 -similar 91 -xdrop 7 -mat 2 -mis -2 -ins -3 -del -3 -mintsd 4 -maxtsd 20 -vic 60 -overlaps best

Additional features within the LTR retrotransposons are annotated by LTRdigest with the following parameters:

-pptradius 30 -pptlen 8 30 -pptrprob 0.97 -uboxlen 3 30 -pptuprob 0.91 -pbsradius 30 -pbsalilen 11 30 -pbsoffset 0 5 -pbstrnaoffset 0 40 -pbsmaxedist 1 -pbsmatchscore 5 -pbsmismatchscore -10 -pbsinsertionscore -20 -pbsdeletionscore -20 -pdomevalcutoff 1e-6 -maxgaplen 50

The subset of candidates that show significant similarity to Pfam protein domains within LTR retrotransposons are selected and then clustered using the ltrclustering program with the following parameters:

-psmall 80 -plarge 30

References