Schema for Inhibit Topoisomerases PolII ChIP-Seq - PolII ChIP-Seq After Inhibition of Topoisomerases
  Database: dm6    Primary Table: GSE47795_PolII_Camp_idr    Row Count: 17,877   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 12987int(10) unsigned range
chromEnd 13381int(10) unsigned range
name .varchar(255) values
score 755int(10) unsigned range
strand .char(1) values
signalValue 2.47844float range
pValue 14.1197float range
qValue 11.7642float range
peak 110int(11) range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValuepeak
585chr2L1298713381.755.2.4784414.119711.7642110
585chr2L4551147451.1000.1.479862.381361.31644252
585chr2L4551147451.1000.2.175877.960236.022641852
585chr2L4551147451.1000.1.637753.529162.2250764
585chr2L4551147451.1000.2.667213.643411.31021117
585chr2L4551147451.1000.2.7118114.657312.278654
585chr2L4551147451.1000.3.2287221.473618.86291359
585chr2L4551147451.1000.4.7026947.282844.25651560
585chr2L4551147451.1000.2.6500613.501411.1774957
585chr2L5265553484.1000.2.070596.905855.0835664

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

Inhibit Topoisomerases PolII ChIP-Seq (GSE47795_polII_chipseq) Track Description
 

Description

These tracks show the regions in the D. melanogaster genome that are significantly enriched in RNA Polymerase II (PolII) in S2 cells as determined by MACS2. The samples were treated with Camptothecin to inhibit topoisomerase I (Top1), and treated with ICRF-193 to inhibit topoisomerase II (Top2). The Irreproducible Discovery Rate (IDR) peak calls were produced by the idr program using a global IDR threshold of 0.05.

The PolII ChIP-Seq datasets were obtained from the NCBI Gene Expression Omnibus database under the accession number GSE47795.

References

Teves SS, Henikoff S. Transcription-generated torsional stress destabilizes nucleosomes. Nat Struct Mol Biol. 2014 Jan;21(1):88-94.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137. doi: 10.1186/gb-2008-9-9-r137. Epub 2008 Sep 17.