Schema for Inhibit Topoisomerases PolII ChIP-Seq - PolII ChIP-Seq After Inhibition of Topoisomerases
  Database: dm6    Primary Table: GSE47795_PolII_ICRF_idr    Row Count: 15,564   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 14013int(10) unsigned range
chromEnd 16025int(10) unsigned range
name .varchar(255) values
score 673int(10) unsigned range
strand .char(1) values
signalValue 1.30534float range
pValue 1.48385float range
qValue 0.63808float range
peak 43int(11) range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValuepeak
585chr2L1401316025.673.1.305341.483850.6380843
585chr2L1401316025.673.1.336161.570370.698251799
585chr2L1401316025.673.1.499162.552881.441331569
585chr2L1401316025.673.1.546312.893881.709981427
585chr2L1401316025.673.1.552643.011.79984439
585chr2L1401316025.673.1.724664.355322.88669974
585chr2L1401316025.673.1.984646.81144.976141239
585chr2L1401316025.673.2.6107714.836412.3712592
585chr2L4594047474.1000.2.282978.971676.90038520
585chr2L4594047474.1000.3.056519.112516.4691244

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

Inhibit Topoisomerases PolII ChIP-Seq (GSE47795_polII_chipseq) Track Description
 

Description

These tracks show the regions in the D. melanogaster genome that are significantly enriched in RNA Polymerase II (PolII) in S2 cells as determined by MACS2. The samples were treated with Camptothecin to inhibit topoisomerase I (Top1), and treated with ICRF-193 to inhibit topoisomerase II (Top2). The Irreproducible Discovery Rate (IDR) peak calls were produced by the idr program using a global IDR threshold of 0.05.

The PolII ChIP-Seq datasets were obtained from the NCBI Gene Expression Omnibus database under the accession number GSE47795.

References

Teves SS, Henikoff S. Transcription-generated torsional stress destabilizes nucleosomes. Nat Struct Mol Biol. 2014 Jan;21(1):88-94.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137. doi: 10.1186/gb-2008-9-9-r137. Epub 2008 Sep 17.