Schema for PePr Histone Modifications Peaks - ChIP-Seq Peaks Identified by PePr
  Database: dm6    Primary Table: H3K27me3_PePr Data last updated: 2016-01-26
Big Bed File: /gbdb/dm6/bbi/H3K27me3_PePr.bb
Item Count: 5,602
Format description: Format used for the output of the PePr peak caller
fieldexampledescription
chromchr2LReference sequence chromosome or scaffold
chromStart16412070Start position in chromosome
chromEnd16412160End position in chromosome
nameH3K27me3_PePr_4403Short Name of item
score37Score from 0-1000
strand++ or -
pValue5.27787Statistical significance of signal value (-log10).
qValue3.77473Statistical significance with multiple-test correction applied (FDR).

Sample Rows
 
chromchromStartchromEndnamescorestrandpValueqValue
chr2L1641207016412160H3K27me3_PePr_440337+5.277873.77473
chr2L1641600016416210H3K27me3_PePr_440447+6.490974.76679
chr2L1641699016417140H3K27me3_PePr_440543+5.921124.30290
chr2L1641843016418760H3K27me3_PePr_440657+7.723725.75109
chr2L1641918016419660H3K27me3_PePr_440768+9.155736.88711
chr2L1641999016421550H3K27me3_PePr_440871+9.422327.10047
chr2L1642176016422720H3K27me3_PePr_440991+11.998869.19060
chr2L1642308016423710H3K27me3_PePr_441088+11.605608.86362
chr2L1642407016425150H3K27me3_PePr_441175+9.925797.50345
chr2L1642623016426980H3K27me3_PePr_441269+9.289706.99375

PePr Histone Modifications Peaks (PePr_peaks) Track Description
 

Description

ChIP-Seq data for H3K4me2, H3K9me2, and H3K27me3 were analyzed using PePr in order to identify regions in the D. melanogaster genome that are enriched for these histone modifications.

Reads from the two replicates for each histone modification were provided to PePr in a single run. Regions enriched in H3K4me2 were identified using PePr with the following parameters:

--peaktype=sharp --remove_artefacts

Regions enriched in H3K9me2 and H3K27me3 were identified using PePr with the following parameters:

--peaktype=broad --remove_artefacts

References

Zhang Y, Lin YH, Johnson TD, Rozek LS, Sartor MA. PePr: a peak-calling prioritization pipeline to identify consistent or differential peaks from replicated ChIP-Seq data. Bioinformatics. 2014 Sep 15;30(18):2568-75.