Schema for Basal Transcription Factors ChIP-exo - ChIP-exo of Basal Transcription Factors in S2R+ Cells
  Database: dm6    Primary Table: M1BP_Baumann2018_macs    Row Count: 3,701   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 18533int(10) unsigned range
chromEnd 18750int(10) unsigned range
name M1BP_Baumann2018_macs_peak_1varchar(255) values
score 1623int(10) unsigned range
strand .char(1) values
signalValue 59.5142float range
pValue 166.239float range
qValue 162.391float range
peak 103int(11) range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValuepeak
585chr2L1853318750M1BP_Baumann2018_macs_peak_11623.59.5142166.239162.391103
585chr2L1885618977M1BP_Baumann2018_macs_peak_2122.9.5464214.801212.203972
585chr2L2137721509M1BP_Baumann2018_macs_peak_3161.5.0226518.830516.16481
586chr2L155964156422M1BP_Baumann2018_macs_peak_41329.44.7039136.647132.974168
586chr2L158836159138M1BP_Baumann2018_macs_peak_51128.44.5441116.4112.855160
586chr2L207258207486M1BP_Baumann2018_macs_peak_6486.17.053551.689748.639106
586chr2L252263252514M1BP_Baumann2018_macs_peak_71004.41.105103.904100.449128
587chr2L277176277642M1BP_Baumann2018_macs_peak_8a704.16.251373.711870.4839127
587chr2L277176277642M1BP_Baumann2018_macs_peak_8b314.16.55234.340431.466361
587chr2L285649285905M1BP_Baumann2018_macs_peak_9629.28.562566.092462.9136136

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

Basal Transcription Factors ChIP-exo (TFChIPexo) Track Description
 

Description

These tracks show the results of the ChIP-exo analyses of the transcription factors that are associated with the core promoters in S2R+ cells, including the Motif 1 Binding Protein (M1BP) and the GST-containing FLYWCH zinc-finger protein (gfzf). To increase the gfzf signal, an additional gfzf sample was crosslinked with dimethyl adipimidate and ethylene glycol bis(succinimidyl succinate), and then with formaldehyde (gfzf_3xlink).

The regions that are enriched in the transcription factors were identified by MACS2, Peakzilla, PeakXus. The log likelihood ratio evidence tracks were produced by MACS2.

The ChIP-exo datasets were obtained from the Gene Expression Omnibus database at NCBI under the accession numbers GSE97841 and GSE105009.

References

Baumann DG, Dai MS, Lu H, Gilmour DS. GFZF, a glutathione S-transferase protein implicated in cell cycle regulation and hybrid inviability, is a transcriptional co-activator. Mol Cell Biol. 2018. Mol Cell Biol 38:e00476-17.

Baumann DG, Gilmour DS. A sequence-specific core promoter-binding transcription factor recruits TRF2 to coordinately transcribe ribosomal protein genes. Nucleic Acids Res. 2017 Oct 13;45(18):10481-10491.

Bardet AF, Steinmann J, Bafna S, Knoblich JA, Zeitlinger J, Stark A. Identification of transcription factor binding sites from ChIP-seq data at high resolution. Bioinformatics. 2013 Nov 1;29(21):2705-13.

Hartonen T, Sahu B, Dave K, Kivioja T, Taipale J. PeakXus: comprehensive transcription factor binding site discovery from ChIP-Nexus and ChIP-Exo experiments. Bioinformatics. 2016 Sep 1;32(17):i629-i638.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137.