Schema for Basal Transcription Factors ChIP-exo - ChIP-exo of Basal Transcription Factors in S2R+ Cells
  Database: dm6    Primary Table: M1BP_Baumann2018_peakxus    Row Count: 31,020   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 11454int(10) unsigned range
chromEnd 11457int(10) unsigned range
name PeakXus_1varchar(255) values
score 29int(10) unsigned range
strand .char(1) values
signalValue 4.02float range
pValue 1.78241float range
qValue 0.60434float range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValue
585chr2L1145411457PeakXus_129.4.021.782410.60434
585chr2L1860518650PeakXus_21000.272307.653307.653
585chr2L1865118683PeakXus_3250.130.021.708410.5414
585chr2L1877818793PeakXus_4104.113.267751.74211
585chr2L1889518921PeakXus_51000.429.043197.18576
585chr2L1893618967PeakXus_6112.32.012.073560.78635
585chr2L1920219213PeakXus_774.7.031.509730.42887
585chr2L2145121491PeakXus_81000.106307.653307.653
585chr2L2169521706PeakXus_957.10.031.559050.46028
585chr2L2170921751PeakXus_10383.19.051.33530.33832

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

Basal Transcription Factors ChIP-exo (TFChIPexo) Track Description
 

Description

These tracks show the results of the ChIP-exo analyses of the transcription factors that are associated with the core promoters in S2R+ cells, including the Motif 1 Binding Protein (M1BP) and the GST-containing FLYWCH zinc-finger protein (gfzf). To increase the gfzf signal, an additional gfzf sample was crosslinked with dimethyl adipimidate and ethylene glycol bis(succinimidyl succinate), and then with formaldehyde (gfzf_3xlink).

The regions that are enriched in the transcription factors were identified by MACS2, Peakzilla, PeakXus. The log likelihood ratio evidence tracks were produced by MACS2.

The ChIP-exo datasets were obtained from the Gene Expression Omnibus database at NCBI under the accession numbers GSE97841 and GSE105009.

References

Baumann DG, Dai MS, Lu H, Gilmour DS. GFZF, a glutathione S-transferase protein implicated in cell cycle regulation and hybrid inviability, is a transcriptional co-activator. Mol Cell Biol. 2018. Mol Cell Biol 38:e00476-17.

Baumann DG, Gilmour DS. A sequence-specific core promoter-binding transcription factor recruits TRF2 to coordinately transcribe ribosomal protein genes. Nucleic Acids Res. 2017 Oct 13;45(18):10481-10491.

Bardet AF, Steinmann J, Bafna S, Knoblich JA, Zeitlinger J, Stark A. Identification of transcription factor binding sites from ChIP-seq data at high resolution. Bioinformatics. 2013 Nov 1;29(21):2705-13.

Hartonen T, Sahu B, Dave K, Kivioja T, Taipale J. PeakXus: comprehensive transcription factor binding site discovery from ChIP-Nexus and ChIP-Exo experiments. Bioinformatics. 2016 Sep 1;32(17):i629-i638.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137.