Schema for Basal Transcription Factors ChIP-exo - ChIP-exo of Basal Transcription Factors in S2R+ Cells
  Database: dm6    Primary Table: M1BP_chipexo_macs    Row Count: 2,453   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 18506int(10) unsigned range
chromEnd 18993int(10) unsigned range
name M1BP_chipexo_macs_peak_1avarchar(255) values
score 706int(10) unsigned range
strand .char(1) values
signalValue 29.8241float range
pValue 73.5627float range
qValue 70.643float range
peak 119int(11) range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValuepeak
585chr2L1850618993M1BP_chipexo_macs_peak_1a706.29.824173.562770.643119
585chr2L1850618993M1BP_chipexo_macs_peak_1b146.9.9553317.203114.6837420
586chr2L155984156397M1BP_chipexo_macs_peak_21314.44.4514134.698131.458153
586chr2L158749159156M1BP_chipexo_macs_peak_32938.90.7699297.79293.822238
586chr2L207243207520M1BP_chipexo_macs_peak_4799.24.108282.969479.9961133
586chr2L252179252565M1BP_chipexo_macs_peak_51539.54.1578157.254153.911211
587chr2L277174277721M1BP_chipexo_macs_peak_6a1134.22.407116.586113.435129
587chr2L277174277721M1BP_chipexo_macs_peak_6b578.23.924960.718757.8806384
587chr2L285646285945M1BP_chipexo_macs_peak_71665.57.5065169.992166.592134
587chr2L292266292532M1BP_chipexo_macs_peak_8441.20.894346.862944.1087134

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

Basal Transcription Factors ChIP-exo (TFChIPexo) Track Description
 

Description

These tracks show the results of the ChIP-exo analyses of the transcription factors that are associated with the core promoters in S2R+ cells, including the Motif 1 Binding Protein (M1BP) and the GST-containing FLYWCH zinc-finger protein (gfzf). To increase the gfzf signal, an additional gfzf sample was crosslinked with dimethyl adipimidate and ethylene glycol bis(succinimidyl succinate), and then with formaldehyde (gfzf_3xlink).

The regions that are enriched in the transcription factors were identified by MACS2, Peakzilla, PeakXus. The log likelihood ratio evidence tracks were produced by MACS2.

The ChIP-exo datasets were obtained from the Gene Expression Omnibus database at NCBI under the accession numbers GSE97841 and GSE105009.

References

Baumann DG, Dai MS, Lu H, Gilmour DS. GFZF, a glutathione S-transferase protein implicated in cell cycle regulation and hybrid inviability, is a transcriptional co-activator. Mol Cell Biol. 2018. Mol Cell Biol 38:e00476-17.

Baumann DG, Gilmour DS. A sequence-specific core promoter-binding transcription factor recruits TRF2 to coordinately transcribe ribosomal protein genes. Nucleic Acids Res. 2017 Oct 13;45(18):10481-10491.

Bardet AF, Steinmann J, Bafna S, Knoblich JA, Zeitlinger J, Stark A. Identification of transcription factor binding sites from ChIP-seq data at high resolution. Bioinformatics. 2013 Nov 1;29(21):2705-13.

Hartonen T, Sahu B, Dave K, Kivioja T, Taipale J. PeakXus: comprehensive transcription factor binding site discovery from ChIP-Nexus and ChIP-Exo experiments. Bioinformatics. 2016 Sep 1;32(17):i629-i638.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137.