Schema for Basal Transcription Factors ChIP-exo - ChIP-exo of Basal Transcription Factors in S2R+ Cells
  Database: dm6    Primary Table: M1BP_chipexo_peakxus    Row Count: 14,024   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 13857int(10) unsigned range
chromEnd 13866int(10) unsigned range
name PeakXus_1varchar(255) values
score 19int(10) unsigned range
strand .char(1) values
signalValue 4.04float range
pValue 1.3867float range
qValue 0.02862float range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValue
585chr2L1385713866PeakXus_119.4.041.38670.02862
585chr2L1861618643PeakXus_21000.100307.653307.653
585chr2L1865318693PeakXus_3196.69.031.602030.16649
585chr2L1870518719PeakXus_455.285.589193.77995
585chr2L1874718751PeakXus_519.11.012.010160.48535
585chr2L1888618916PeakXus_6430.233.600161.90494
585chr2L1892218924PeakXus_717.7.012.218350.6615
585chr2L1893418964PeakXus_876.24.012.06930.53486
585chr2L2146021500PeakXus_9738.3411.05629.0623
585chr2L2174721749PeakXus_1036.8.041.423920.05269

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

Basal Transcription Factors ChIP-exo (TFChIPexo) Track Description
 

Description

These tracks show the results of the ChIP-exo analyses of the transcription factors that are associated with the core promoters in S2R+ cells, including the Motif 1 Binding Protein (M1BP) and the GST-containing FLYWCH zinc-finger protein (gfzf). To increase the gfzf signal, an additional gfzf sample was crosslinked with dimethyl adipimidate and ethylene glycol bis(succinimidyl succinate), and then with formaldehyde (gfzf_3xlink).

The regions that are enriched in the transcription factors were identified by MACS2, Peakzilla, PeakXus. The log likelihood ratio evidence tracks were produced by MACS2.

The ChIP-exo datasets were obtained from the Gene Expression Omnibus database at NCBI under the accession numbers GSE97841 and GSE105009.

References

Baumann DG, Dai MS, Lu H, Gilmour DS. GFZF, a glutathione S-transferase protein implicated in cell cycle regulation and hybrid inviability, is a transcriptional co-activator. Mol Cell Biol. 2018. Mol Cell Biol 38:e00476-17.

Baumann DG, Gilmour DS. A sequence-specific core promoter-binding transcription factor recruits TRF2 to coordinately transcribe ribosomal protein genes. Nucleic Acids Res. 2017 Oct 13;45(18):10481-10491.

Bardet AF, Steinmann J, Bafna S, Knoblich JA, Zeitlinger J, Stark A. Identification of transcription factor binding sites from ChIP-seq data at high resolution. Bioinformatics. 2013 Nov 1;29(21):2705-13.

Hartonen T, Sahu B, Dave K, Kivioja T, Taipale J. PeakXus: comprehensive transcription factor binding site discovery from ChIP-Nexus and ChIP-Exo experiments. Bioinformatics. 2016 Sep 1;32(17):i629-i638.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137.