Schema for Basal Transcription Factors ChIP-exo - ChIP-exo of Basal Transcription Factors in S2R+ Cells
  Database: dm6    Primary Table: TAF1_chipexo_macs    Row Count: 2,954   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 72382int(10) unsigned range
chromEnd 72732int(10) unsigned range
name TAF1_chipexo_macs_peak_1avarchar(255) values
score 34int(10) unsigned range
strand .char(1) values
signalValue 4.83361float range
pValue 5.80611float range
qValue 3.47937float range
peak 97int(11) range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValuepeak
585chr2L7238272732TAF1_chipexo_macs_peak_1a34.4.833615.806113.4793797
585chr2L7238272732TAF1_chipexo_macs_peak_1b123.8.5994614.960612.3001261
585chr2L8724387497TAF1_chipexo_macs_peak_2582.25.873261.492258.2756127
585chr2L107996108308TAF1_chipexo_macs_peak_32105.58.2485214.484210.575160
585chr2L120336121113TAF1_chipexo_macs_peak_4490.19.452252.222249.0805251
585chr2L121657121817TAF1_chipexo_macs_peak_5487.15.697151.938848.79871
585chr2L129047129433TAF1_chipexo_macs_peak_610295.251.2111034.861029.58187
586chr2L131820132269TAF1_chipexo_macs_peak_72001.65.5131203.975200.1293
586chr2L143262143441TAF1_chipexo_macs_peak_8140.10.017316.788614.074185
586chr2L158926159338TAF1_chipexo_macs_peak_91266.46.303130.25126.656127

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

Basal Transcription Factors ChIP-exo (TFChIPexo) Track Description
 

Description

These tracks show the results of the ChIP-exo analyses of the transcription factors that are associated with the core promoters in S2R+ cells, including the Motif 1 Binding Protein (M1BP) and the GST-containing FLYWCH zinc-finger protein (gfzf). To increase the gfzf signal, an additional gfzf sample was crosslinked with dimethyl adipimidate and ethylene glycol bis(succinimidyl succinate), and then with formaldehyde (gfzf_3xlink).

The regions that are enriched in the transcription factors were identified by MACS2, Peakzilla, PeakXus. The log likelihood ratio evidence tracks were produced by MACS2.

The ChIP-exo datasets were obtained from the Gene Expression Omnibus database at NCBI under the accession numbers GSE97841 and GSE105009.

References

Baumann DG, Dai MS, Lu H, Gilmour DS. GFZF, a glutathione S-transferase protein implicated in cell cycle regulation and hybrid inviability, is a transcriptional co-activator. Mol Cell Biol. 2018. Mol Cell Biol 38:e00476-17.

Baumann DG, Gilmour DS. A sequence-specific core promoter-binding transcription factor recruits TRF2 to coordinately transcribe ribosomal protein genes. Nucleic Acids Res. 2017 Oct 13;45(18):10481-10491.

Bardet AF, Steinmann J, Bafna S, Knoblich JA, Zeitlinger J, Stark A. Identification of transcription factor binding sites from ChIP-seq data at high resolution. Bioinformatics. 2013 Nov 1;29(21):2705-13.

Hartonen T, Sahu B, Dave K, Kivioja T, Taipale J. PeakXus: comprehensive transcription factor binding site discovery from ChIP-Nexus and ChIP-Exo experiments. Bioinformatics. 2016 Sep 1;32(17):i629-i638.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137.