Schema for Basal Transcription Factors ChIP-exo - ChIP-exo of Basal Transcription Factors in S2R+ Cells
  Database: dm6    Primary Table: gfzf_3x_Baumann2018_macs    Row Count: 2,420   Data last updated: 2022-10-21
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 18519int(10) unsigned range
chromEnd 18714int(10) unsigned range
name GFZF_3x_Baumann2018_macs_pe...varchar(255) values
score 584int(10) unsigned range
strand .char(1) values
signalValue 23.1561float range
pValue 61.7675float range
qValue 58.4458float range
peak 110int(11) range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValuepeak
585chr2L1851918714GFZF_3x_Baumann2018_macs_peak_1584.23.156161.767558.4458110
585chr2L1886318995GFZF_3x_Baumann2018_macs_peak_229.4.16815.432732.95736103
585chr2L121023121164GFZF_3x_Baumann2018_macs_peak_346.5.030997.254864.698172
586chr2L131794132103GFZF_3x_Baumann2018_macs_peak_490.5.4775511.75149.0477674
586chr2L156001156236GFZF_3x_Baumann2018_macs_peak_5682.22.341371.692268.2899123
586chr2L158839159142GFZF_3x_Baumann2018_macs_peak_6817.21.942485.279281.7648142
586chr2L207281207513GFZF_3x_Baumann2018_macs_peak_7189.9.0285721.875118.9854109
586chr2L252287252443GFZF_3x_Baumann2018_macs_peak_8279.13.893730.985227.973695
587chr2L277171277617GFZF_3x_Baumann2018_macs_peak_9a387.15.364641.847338.7166146
587chr2L277171277617GFZF_3x_Baumann2018_macs_peak_9b147.9.2624317.581414.7599382

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

Basal Transcription Factors ChIP-exo (TFChIPexo) Track Description
 

Description

These tracks show the results of the ChIP-exo analyses of the transcription factors that are associated with the core promoters in S2R+ cells, including the Motif 1 Binding Protein (M1BP) and the GST-containing FLYWCH zinc-finger protein (gfzf). To increase the gfzf signal, an additional gfzf sample was crosslinked with dimethyl adipimidate and ethylene glycol bis(succinimidyl succinate), and then with formaldehyde (gfzf_3xlink).

The regions that are enriched in the transcription factors were identified by MACS2, Peakzilla, PeakXus. The log likelihood ratio evidence tracks were produced by MACS2.

The ChIP-exo datasets were obtained from the Gene Expression Omnibus database at NCBI under the accession numbers GSE97841 and GSE105009.

References

Baumann DG, Dai MS, Lu H, Gilmour DS. GFZF, a glutathione S-transferase protein implicated in cell cycle regulation and hybrid inviability, is a transcriptional co-activator. Mol Cell Biol. 2018. Mol Cell Biol 38:e00476-17.

Baumann DG, Gilmour DS. A sequence-specific core promoter-binding transcription factor recruits TRF2 to coordinately transcribe ribosomal protein genes. Nucleic Acids Res. 2017 Oct 13;45(18):10481-10491.

Bardet AF, Steinmann J, Bafna S, Knoblich JA, Zeitlinger J, Stark A. Identification of transcription factor binding sites from ChIP-seq data at high resolution. Bioinformatics. 2013 Nov 1;29(21):2705-13.

Hartonen T, Sahu B, Dave K, Kivioja T, Taipale J. PeakXus: comprehensive transcription factor binding site discovery from ChIP-Nexus and ChIP-Exo experiments. Bioinformatics. 2016 Sep 1;32(17):i629-i638.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137.