Schema for PolII ChIP-Seq (Cell Lines) - RNA Polymerase II ChIP-Seq for Cell Lines
  Database: dm6    Primary Table: EMBL_PolII_S2_idr    Row Count: 32,233   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 5533int(10) unsigned range
chromEnd 6013int(10) unsigned range
name .varchar(255) values
score 1000int(10) unsigned range
strand .char(1) values
signalValue 2.90109float range
pValue 30.2774float range
qValue 28.7036float range
peak 317int(11) range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValuepeak
585chr2L55336013.1000.2.9010930.277428.7036317
585chr2L76488379.629.1.902227.388696.24101609
585chr2L76488379.629.1.93128.840797.65218310
585chr2L86929458.866.2.3029517.538516.1607506
585chr2L86929458.866.2.2218118.393617.0004195
585chr2L1822619215.1000.3.3601725.551624.0442392
585chr2L1822619215.1000.1.517562.799691.8247898
585chr2L1822619215.1000.2.6398317.571816.1933109
585chr2L1822619215.1000.3.093523.014121.5447672
585chr2L6667267719.1000.3.5089475.812173.67390

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

PolII ChIP-Seq (Cell Lines) (cells_polII_chipseq) Track Description
 

Description

These tracks show the regions in the D. melanogaster genome that are significantly enriched in RNA Polymerase II (PolII) in different cell lines as determined by MACS2. The signal tracks correspond to the log likelihood enrichment ratios of the treatment versus the DNA input sample as determined by the bdgcmp program in MACS2. The Irreproducible Discovery Rate (IDR) peak calls were produced by the idr program using a global IDR threshold of 0.05.

The RNA PolII ChIP-Seq reads for the S2 cells were obtained from the EBI ENA database under the accession number PRJEB3031. The antibody used in this study recognizes the Rpb3 subunit of RNA PolII.

References

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137. doi: 10.1186/gb-2008-9-9-r137. Epub 2008 Sep 17.