Schema for PolII ChIP-Seq (Cell Lines) - RNA Polymerase II ChIP-Seq for Cell Lines
  Database: dm6    Primary Table: EMBL_PolII_S2_logLR    Row Count: 1   Data last updated: 2022-10-20
fieldexampleSQL type info
fileName /gbdb/dm6/bbi/PolII_S2_macs...varchar(255) values

Sample Rows
 
fileName
/gbdb/dm6/bbi/PolII_S2_macs_logLR.bw

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

PolII ChIP-Seq (Cell Lines) (cells_polII_chipseq) Track Description
 

Description

These tracks show the regions in the D. melanogaster genome that are significantly enriched in RNA Polymerase II (PolII) in different cell lines as determined by MACS2. The signal tracks correspond to the log likelihood enrichment ratios of the treatment versus the DNA input sample as determined by the bdgcmp program in MACS2. The Irreproducible Discovery Rate (IDR) peak calls were produced by the idr program using a global IDR threshold of 0.05.

The RNA PolII ChIP-Seq reads for the S2 cells were obtained from the EBI ENA database under the accession number PRJEB3031. The antibody used in this study recognizes the Rpb3 subunit of RNA PolII.

References

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137. doi: 10.1186/gb-2008-9-9-r137. Epub 2008 Sep 17.