Schema for PolII ChIP-Seq (Cell Lines) - RNA Polymerase II ChIP-Seq for Cell Lines
  Database: dm6    Primary Table: EMBL_PolII_S2_macs    Row Count: 40,156   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 5583int(10) unsigned range
chromEnd 5999int(10) unsigned range
name PolII_S2_macs_peak_1varchar(255) values
score 287int(10) unsigned range
strand .char(1) values
signalValue 2.90109float range
pValue 30.2774float range
qValue 28.7036float range
peak 267int(11) range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValuepeak
585chr2L55835999PolII_S2_macs_peak_1287.2.9010930.277428.7036267
585chr2L77388055PolII_S2_macs_peak_276.1.93128.840797.65218220
585chr2L87139422PolII_S2_macs_peak_3a170.2.2218118.393617.0004174
585chr2L87139422PolII_S2_macs_peak_3b161.2.3029517.538516.1607485
585chr2L1177011967PolII_S2_macs_peak_492.2.0753110.52929.2973108
585chr2L1207012242PolII_S2_macs_peak_540.1.661535.133844.06006105
585chr2L1234213032PolII_S2_macs_peak_6a147.2.2237616.127914.776137
585chr2L1234213032PolII_S2_macs_peak_6b94.2.0631210.67139.43621487
585chr2L1349614113PolII_S2_macs_peak_7a30.1.638894.114443.0801380
585chr2L1349614113PolII_S2_macs_peak_7b122.2.1197213.588212.2872458

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

PolII ChIP-Seq (Cell Lines) (cells_polII_chipseq) Track Description
 

Description

These tracks show the regions in the D. melanogaster genome that are significantly enriched in RNA Polymerase II (PolII) in different cell lines as determined by MACS2. The signal tracks correspond to the log likelihood enrichment ratios of the treatment versus the DNA input sample as determined by the bdgcmp program in MACS2. The Irreproducible Discovery Rate (IDR) peak calls were produced by the idr program using a global IDR threshold of 0.05.

The RNA PolII ChIP-Seq reads for the S2 cells were obtained from the EBI ENA database under the accession number PRJEB3031. The antibody used in this study recognizes the Rpb3 subunit of RNA PolII.

References

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137. doi: 10.1186/gb-2008-9-9-r137. Epub 2008 Sep 17.