Schema for MUSIC Histone Modifications Peaks - ChIP-Seq Peaks Identified by MUSIC

Database: dm6 Primary Table: MUSIC_H3K27me3 Data last updated: 2016-01-27
Big Bed File: /gbdb/dm6/bbi/MUSIC_H3K27me3_ERs.bb
Item Count: 428
Format description: BED6+3 Peaks of signal enrichment based on pooled, normalized (interpreted) data.

field	example	description
`chrom`	chr2L	Reference sequence chromosome or scaffold
`chromStart`	16365917	Start position in chromosome
`chromEnd`	16371838	End position in chromosome
`name`	MUSIC_H3K27me3_ERs_49	Name given to a region (preferably unique). Use . if no name is assigned.
`score`	12	Indicates how dark the peak will be displayed in the browser (0-1000)
`strand`	+	+ or - or . for unknown
`signalValue`	1	Measurement of average enrichment for the region
`pValue`	0.16100	Statistical significance of signal value (-log10). Set to -1 if not used.
`qValue`	0.12231	Statistical significance with multiple-test correction applied (FDR -log10). Set to -1 if not used.

Sample Rows

chrom	chromStart	chromEnd	name	score	strand	signalValue	pValue	qValue
chr2L	16365917	16371838	MUSIC_H3K27me3_ERs_49	12	+	1	0.16100	0.12231
chr2L	16378761	16490155	MUSIC_H3K27me3_ERs_50	59	+	2	0.66081	0.59714
chr2L	17246095	17271993	MUSIC_H3K27me3_ERs_51	15	+	1	0.21935	0.15047
chr2L	17735818	17782547	MUSIC_H3K27me3_ERs_52	13	+	1	0.19135	0.13124
chr2L	18715510	18762631	MUSIC_H3K27me3_ERs_53	24	+	2	0.33446	0.24667
chr2L	18763504	18828621	MUSIC_H3K27me3_ERs_54	87	+	3	0.92003	0.87522
chr2L	18859923	18937160	MUSIC_H3K27me3_ERs_55	110	+	4	1.14072	1.10206
chr2L	19070061	19114014	MUSIC_H3K27me3_ERs_56	23	+	2	0.32147	0.23504
chr2L	19234154	19235214	MUSIC_H3K27me3_ERs_57	11	+	1	0.14267	0.11607
chr2L	19324684	19328715	MUSIC_H3K27me3_ERs_58	14	+	1	0.20362	0.14179

MUSIC Histone Modifications Peaks (chipseq_MUSIC_peaks) Track Description


	Description ChIP-Seq data for H3K4me2, H3K9me2, and H3K27me3 were analyzed using MUSIC in order to identify regions in the D. melanogaster genome that are enriched for these histone modifications. Reads from the two replicates were merged into a single file prior to the analysis with MUSIC. Only the subset of scaffolds larger than 100kb in the D. melanogaster assembly were analyzed by MUSIC. Broad peaks for H3K9me2 and H3K27me3 were identified using the -get_optimal_broad_ERs option. Punctated peaks for H3K4me2 were identified using the -get_optimal_punctate_ERs option. References Harmanci A, Rozowsky J, Gerstein M. MUSIC: identification of enriched regions in ChIP-Seq experiments using a mappability-corrected multiscale signal processing framework. Genome Biol. 2014;15(10):474.

Description

References