Schema for MUSIC Histone Modifications Peaks - ChIP-Seq Peaks Identified by MUSIC

Database: dm6 Primary Table: MUSIC_H3K9me2 Data last updated: 2016-01-27
Big Bed File: /gbdb/dm6/bbi/MUSIC_H3K9me2_ERs.bb
Item Count: 1,200
Format description: BED6+3 Peaks of signal enrichment based on pooled, normalized (interpreted) data.

field	example	description
`chrom`	chr2L	Reference sequence chromosome or scaffold
`chromStart`	15683871	Start position in chromosome
`chromEnd`	15686799	End position in chromosome
`name`	MUSIC_H3K9me2_ERs_121	Name given to a region (preferably unique). Use . if no name is assigned.
`score`	52	Indicates how dark the peak will be displayed in the browser (0-1000)
`strand`	+	+ or - or . for unknown
`signalValue`	2	Measurement of average enrichment for the region
`pValue`	0.58318	Statistical significance of signal value (-log10). Set to -1 if not used.
`qValue`	0.52124	Statistical significance with multiple-test correction applied (FDR -log10). Set to -1 if not used.

Sample Rows

chrom	chromStart	chromEnd	name	score	strand	signalValue	pValue	qValue
chr2L	15683871	15686799	MUSIC_H3K9me2_ERs_121	52	+	2	0.58318	0.52124
chr2L	15718948	15724067	MUSIC_H3K9me2_ERs_122	19	+	1	0.24415	0.19713
chr2L	15826920	15829242	MUSIC_H3K9me2_ERs_123	99	+	2	1.06713	0.99936
chr2L	16141175	16146397	MUSIC_H3K9me2_ERs_124	35	+	1	0.42723	0.35935
chr2L	16243343	16244943	MUSIC_H3K9me2_ERs_125	12	+	1	0.13314	0.12494
chr2L	16303272	16304675	MUSIC_H3K9me2_ERs_126	37	+	1	0.44293	0.37522
chr2L	16329767	16336198	MUSIC_H3K9me2_ERs_127	13	+	1	0.15465	0.13635
chr2L	16512190	16518871	MUSIC_H3K9me2_ERs_128	108	+	3	1.16035	1.08493
chr2L	16557763	16559733	MUSIC_H3K9me2_ERs_129	42	+	2	0.49091	0.42455
chr2L	16779389	16790582	MUSIC_H3K9me2_ERs_130	22	+	2	0.27937	0.22485

MUSIC Histone Modifications Peaks (chipseq_MUSIC_peaks) Track Description


	Description ChIP-Seq data for H3K4me2, H3K9me2, and H3K27me3 were analyzed using MUSIC in order to identify regions in the D. melanogaster genome that are enriched for these histone modifications. Reads from the two replicates were merged into a single file prior to the analysis with MUSIC. Only the subset of scaffolds larger than 100kb in the D. melanogaster assembly were analyzed by MUSIC. Broad peaks for H3K9me2 and H3K27me3 were identified using the -get_optimal_broad_ERs option. Punctated peaks for H3K4me2 were identified using the -get_optimal_punctate_ERs option. References Harmanci A, Rozowsky J, Gerstein M. MUSIC: identification of enriched regions in ChIP-Seq experiments using a mappability-corrected multiscale signal processing framework. Genome Biol. 2014;15(10):474.

Description

References