Schema for MUSIC Histone Modifications Peaks - ChIP-Seq Peaks Identified by MUSIC
  Database: dm6    Primary Table: punctate_MUSIC_H3K4me2 Data last updated: 2016-01-27
Big Bed File: /gbdb/dm6/bbi/punctate_MUSIC_H3K4me2_ERs.bb
Item Count: 5,418
Format description: BED6+3 Peaks of signal enrichment based on pooled, normalized (interpreted) data.
fieldexampledescription
chromchr2LReference sequence chromosome or scaffold
chromStart15730616Start position in chromosome
chromEnd15732120End position in chromosome
namepunctate_MUSIC_H3K4me2_ERs_648Name given to a region (preferably unique). Use . if no name is assigned.
score130Indicates how dark the peak will be displayed in the browser (0-1000)
strand++ or - or . for unknown
signalValue2Measurement of average enrichment for the region
pValue1.30955Statistical significance of signal value (-log10). Set to -1 if not used.
qValue1.30412Statistical significance with multiple-test correction applied (FDR -log10). Set to -1 if not used.

Sample Rows
 
chromchromStartchromEndnamescorestrandsignalValuepValueqValue
chr2L1573061615732120punctate_MUSIC_H3K4me2_ERs_648130+21.309551.30412
chr2L1574413615748235punctate_MUSIC_H3K4me2_ERs_649193+21.938291.93650
chr2L1575040115753540punctate_MUSIC_H3K4me2_ERs_650216+32.166042.16451
chr2L1576030015771178punctate_MUSIC_H3K4me2_ERs_651209+22.100072.09855
chr2L1623179016232107punctate_MUSIC_H3K4me2_ERs_65218+10.212180.18355
chr2L1624990816259065punctate_MUSIC_H3K4me2_ERs_653221+22.216852.21529
chr2L1626080816262972punctate_MUSIC_H3K4me2_ERs_654214+32.150462.14893
chr2L1628512916289402punctate_MUSIC_H3K4me2_ERs_655224+22.250522.24893
chr2L1629852716311876punctate_MUSIC_H3K4me2_ERs_656228+32.290092.28845
chr2L1632372316329121punctate_MUSIC_H3K4me2_ERs_657229+32.298242.29659

MUSIC Histone Modifications Peaks (chipseq_MUSIC_peaks) Track Description
 

Description

ChIP-Seq data for H3K4me2, H3K9me2, and H3K27me3 were analyzed using MUSIC in order to identify regions in the D. melanogaster genome that are enriched for these histone modifications.

Reads from the two replicates were merged into a single file prior to the analysis with MUSIC. Only the subset of scaffolds larger than 100kb in the D. melanogaster assembly were analyzed by MUSIC. Broad peaks for H3K9me2 and H3K27me3 were identified using the -get_optimal_broad_ERs option. Punctated peaks for H3K4me2 were identified using the -get_optimal_punctate_ERs option.

References

Harmanci A, Rozowsky J, Gerstein M. MUSIC: identification of enriched regions in ChIP-Seq experiments using a mappability-corrected multiscale signal processing framework. Genome Biol. 2014;15(10):474.