Schema for scRNAs (S2 Cells) - Short Capped RNAs (scRNAs) in S2 Cells
  Database: dm6    Primary Table: scRNA3pr_S2_chromatin_tagClusters    Row Count: 28,646   Data last updated: 2022-10-21
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 22310int(10) unsigned range
chromEnd 22324int(10) unsigned range
name .varchar(255) values
score 0int(10) unsigned range
strand +char(1) values
thickStart 22314int(10) unsigned range
thickEnd 22315int(10) unsigned range
reserved 0int(10) unsigned range
blockCount 1int(10) unsigned range
blockSizes 14longblob  
chromStarts 0longblob  

Sample Rows
 
binchromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStarts
585chr2L2231022324.0+223142231501140
585chr2L4755147553.0+47551475520120
585chr2L5920459210.0-59204592050160
585chr2L6673466735.0-66734667350110
585chr2L6707467123.0+6707767078031,29,10,2,48
585chr2L6714467151.0-67150671510170
585chr2L6723967257.0-672566725701180
585chr2L6767667692.0+676906769101160
585chr2L6931669323.0-69316693170170
585chr2L6947269489.0+6947369474031,14,10,1,16

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

scRNAs (S2 Cells) (scRNAs_S2) Track Description
 

Description

These tracks show the normalized read density for the 5′ and 3′ ends of short capped RNAs (scRNAs) in D. melanogaster S2 cells. Most of the scRNAs are produced by paused polymerases. The 5′ ends of the scRNAs demarcate the transcription start site while the 3′ ends of the scRNAs demarcate where RNA Polymerase II have paused.

Datasets from Nechaev S et al., 2010

This study analyzed the 5′ and 3′ scRNAs found in untreated S2 cells and in TFIIS-depleted S2 cells. The datasets were retrieved from the Gene Expression Omnibus database at NCBI under the accession number GSE18643.

Datasets from Henriques T et al., 2013

In addition to analyzing the 3′ scRNAs found in whole S2 cells, this study also analyzed the 3′ scRNAs that are associated with chromatin and 3′ scRNAs that are detergent-soluble. The datasets were retrieved from the Gene Expression Omnibus database at NCBI under the accession number GSE49078.

Methods

Each dataset was mapped to the D. melanogaster release 6 assembly using bwa with default parameters. The alignment results were analyzed by CAGEr.

For the Nechaev S et al. study, the tag counts were normalized by the normalizeTagCount function in CAGEr with the following parameters:

method = "powerLaw", fitInRange = c(10, 1000), alpha = 1.10, T = 1*10^6

For the Henriques T et al. study, the tag counts were normalized by the normalizeTagCount function in CAGEr with the following parameters:

method = "powerLaw", fitInRange = c(10, 1000), alpha = 1.05, T = 1*10^7

Note that the orientation of the features identified by the CAGEr analysis of the 3′ scRNAs were flipped to facilitate data interpretations.

References

Nechaev S, Fargo DC, dos Santos G, Liu L, Gao Y, Adelman K. Global analysis of short RNAs reveals widespread promoter-proximal stalling and arrest of Pol II in Drosophila. Science. 2010 Jan 15;327(5963):335-8.

Henriques T, Gilchrist DA, Nechaev S, Bern M, Muse GW, Burkholder A, Fargo DC, Adelman K. Stable pausing by RNA polymerase II provides an opportunity to target and integrate regulatory signals. Mol Cell. 2013 Nov 21;52(4):517-28.

Haberle V, Forrest AR, Hayashizaki Y, Carninci P, Lenhard B. CAGEr: precise TSS data retrieval and high-resolution promoterome mining for integrative analyses. Nucleic Acids Res. 2015 Apr 30;43(8):e51.