Schema for CapSeq Tag Clusters - CapSeq Tag Clusters for w1118 (wildtype) Ovaries
  Database: dm6    Primary Table: tss_CapSeq_tagClusters    Row Count: 12,920   Data last updated: 2022-10-21
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 14549int(10) unsigned range
chromEnd 14551int(10) unsigned range
name .varchar(255) values
score 0int(10) unsigned range
strand -char(1) values
thickStart 14549int(10) unsigned range
thickEnd 14550int(10) unsigned range
reserved 0int(10) unsigned range
blockCount 1int(10) unsigned range
blockSizes 2longblob  
chromStarts 0longblob  

Sample Rows
 
binchromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStarts
585chr2L1454914551.0-14549145500120
585chr2L6704367046.0+67043670440130
585chr2L6743467445.0+674446744501110
585chr2L6852768529.0+68527685280120
585chr2L7259972600.0+72599726000110
585chr2L7338773407.0+733877338801200
585chr2L7343873441.0+73440734410130
585chr2L7346873479.0+734727347301110
585chr2L7352073529.0+73522735230190
585chr2L7408874105.0+741047410501170

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

CapSeq Tag Clusters (tss_CapSeq_tagClusters) Track Description
 

Description

These tracks show the interquantile widths of the promoters for D. melanogaster based on the CapSeq data for Drosophila adult ovaries (genotype w1118.tm3). The CapSeq datasets were obtained from the NCBI Sequence Read Archive database under the accession number SRP103849.

Methods

Each dataset was mapped to the D. melanogaster release 6 assembly using bwa with default parameters. Only the alignments with mapping quality of 5 or above are kept. The alignment results were analyzed by CAGEr. The tag counts were normalized by the normalizeTagCount function in CAGEr with the following parameters:

method = "powerLaw", fitInRange = c(10, 1000), alpha = 1.05, T = 1*10^6

The promoter widths were determined by the cumulativeCTSSdistribution and quantilePositions functions in CAGEr using the following parameters:

clusters = "tagClusters", qLow = 0.1, qUp = 0.9

Display Conventions

The span of each feature corresponds to the promoter width. The thin box within each span corresponds to the interquantile width (qLow = 0.1 to qUp = 0.9) that captures 80% of the CapSeq signal. The thicker box denotes the position within the promoter with the highest CapSeq signal.

Credits

Brennecke J, Aravin AA, Stark A, Dus M, Kellis M, Sachidanandam R, Hannon GJ. Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila. Cell. 2007 Mar 23;128(6):1089-103.

Haberle V, Forrest AR, Hayashizaki Y, Carninci P, Lenhard B. CAGEr: precise TSS data retrieval and high-resolution promoterome mining for integrative analyses. Nucleic Acids Res. 2015 Apr 30;43(8):e51.