Description
These tracks show the transcription start sites identified by 5' Massive Amplification of
cDNA Ends (5' MACE) in D. melanogaster S2-DRSC cells and 0-16 hour embryos. The
MACE datasets were obtained from the NCBI Gene Expression Omnibus database under the
accession number GSE24521.
Methods
Each dataset was mapped to the D. melanogaster release 6 assembly using
bwa with default parameters. The alignment
results were analyzed by CAGEr.
The tag counts were normalized by the normalizeTagCount function in CAGEr with the following parameters:
method = "powerLaw", fitInRange = c(10, 1000), alpha = 1.05, T = 2*10^6
References
Enderle D, Beisel C, Stadler MB, Gerstung M, Athri P, Paro R.
Polycomb preferentially targets stalled promoters of coding and noncoding transcripts. Genome Res. 2011 Feb;21(2):216-26.
Haberle V, Forrest AR, Hayashizaki Y, Carninci P, Lenhard B.
CAGEr: precise TSS data retrieval and high-resolution promoterome mining for integrative analyses.
Nucleic Acids Res. 2015 Apr 30;43(8):e51.