Schema for TSRchitect Combined RAMPAGE TSS (Replicate 2) - TSRchitect Combined RAMPAGE Transcription Start Sites (Replicate 2)
  Database: dm6    Primary Table: tss_RAMPAGE2_dedup_Peaks Data last updated: 2021-04-18
Big Bed File: /gbdb/dm6/bbi/RAMPAGE_dedup/GSE89299/GSE89299_tsrchitectPeaks.bb
Item Count: 25,501
Format description: RAMPAGE Peaks Identified by TSRchitect
fieldexampledescription
chromchr2LReference sequence chromosome or scaffold
chromStart15728320Start position in chromosome
chromEnd15728321End position in chromosome
nametsr_peaked_3174Name of RAMPAGE peak
score1000Score from 0-1000
strand-+ or -
nTSSs1Count of TSSs
nTAGs37Count of tags
tsrPeak1.00Maximum tag count fraction for all TSS positions in the TSR
tsrWdth1Width of TSR
tsrTrq0.00TSR torque; measure of TSR balance
tsrSI2.00Shape index (SI) value of TSR
tsrMSI1.00Modified shape index (MSI) value of TSR

Sample Rows
 
chromchromStartchromEndnamescorestrandnTSSsnTAGstsrPeaktsrWdthtsrTrqtsrSItsrMSI
chr2L1572832015728321tsr_peaked_31741000-1371.0010.002.001.00
chr2L1572907715729080tsr_peaked_3175530-2530.533-0.061.000.00
chr2L1572910915729110tsr_peaked_31761000-1261.0010.002.001.00
chr2L1572912915729156tsr_peaked_3177470-52890.47270.55-0.010.13
chr2L1572929715729298tsr_peaked_31781000-1311.0010.002.001.00
chr2L1572934815729354tsr_peaked_3179520-21200.5260.081.000.00
chr2L1572949915729500tsr_peaked_31801000-1381.0010.002.001.00
chr2L1573176815731825tsr_peaked_3181250-14118160.2557-15.93-0.740.28
chr2L1574584115745842tsr_peaked_13661000+1661.0010.002.001.00
chr2L1574651315746525tsr_peaked_3182470-31400.47122.280.470.04

TSRchitect Combined RAMPAGE TSS (Replicate 2) (tss_RAMPAGE2_dedup_Combined) Track Description
 

Description

This track shows the locations of putative Transcription Start Sites (TSS) in the D. melanogaster genome based on the analysis of RAMPAGE results from multiple developmental stages of D. melanogaster embryos.

The datasets were retrieved from the Gene Expression Omnibus database at NCBI under the accession number GSE89299. The RAMPAGE reads were mapped against the D. melanogaster (dm6) assembly using STAR. The putative promoter regions were identified by TSRchitect.

References

Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, Batut P, Chaisson M, Gingeras TR. STAR: ultrafast universal RNA-seq aligner. Bioinformatics. 2013 Jan 1;29(1):15-21.

Batut PJ, Gingeras TR. Conserved noncoding transcription and core promoter regulatory code in early Drosophila development. Elife. 2017 Dec 20;6.

Batut P, Dobin A, Plessy C, Carninci P, Gingeras TR. High-fidelity promoter profiling reveals widespread alternative promoter usage and transposon-driven developmental gene expression. Genome Res. 2013 Jan;23(1):169-80.