Schema for TSRchitect RAMPAGE Embryos TSS - TSRchitect RAMPAGE Embryos Transcription Start Sites
  Database: dm6    Primary Table: tss_RAMPAGE_Dmel05_E05h Data last updated: 2021-04-19
Big Bed File: /gbdb/dm6/bbi/RAMPAGE_dedup/tsrchitect/Dmel05_E05h_tsrchitect.bb
Item Count: 5,749
Format description: RAMPAGE Peaks Identified by TSRchitect
fieldexampledescription
chromchr2LReference sequence chromosome or scaffold
chromStart15731775Start position in chromosome
chromEnd15731825End position in chromosome
nametsr_peaked_831Name of RAMPAGE peak
score260Score from 0-1000
strand-+ or -
nTSSs10Count of TSSs
nTAGs3299Count of tags
tsrPeak0.26Maximum tag count fraction for all TSS positions in the TSR
tsrWdth50Width of TSR
tsrTrq-18.73TSR torque; measure of TSR balance
tsrSI-0.61Shape index (SI) value of TSR
tsrMSI0.21Modified shape index (MSI) value of TSR

Sample Rows
 
chromchromStartchromEndnamescorestrandnTSSsnTAGstsrPeaktsrWdthtsrTrqtsrSItsrMSI
chr2L1573177515731825tsr_peaked_831260-1032990.2650-18.73-0.610.21
chr2L1574811715748123tsr_peaked_832850-21700.8561.741.380.38
chr2L1574814215748143tsr_peaked_8331000-1571.0010.002.001.00
chr2L1575103515751036tsr_peaked_8341000-1311.0010.002.001.00
chr2L1575106715751075tsr_peaked_835710-21240.718-1.471.130.13
chr2L1575227415752310tsr_peaked_374510+31320.51360.100.510.06
chr2L1576830015768301tsr_peaked_8361000-1351.0010.002.001.00
chr2L1625759016257601tsr_peaked_837540-2680.5411-0.441.010.01
chr2L1626169216261723tsr_peaked_838410-52270.4131-7.11-0.120.09
chr2L1628770916287732tsr_peaked_839480-35870.48237.600.700.18

TSRchitect RAMPAGE Embryos TSS (tss_RAMPAGE_embryos) Track Description
 

Description

This track shows the locations of putative Transcription Start Sites (TSS) in the D. melanogaster genome based on the analysis of RAMPAGE data from different embryonic stages in one-hour intervals.

The embryos RAMPAGE datasets were retrieved from the Gene Expression Omnibus database at NCBI under the accession numbers GSE36212 and GSE89299. The RAMPAGE reads were mapped against the D. melanogaster (dm6) assembly using STAR. The putative promoter regions were identified by TSRchitect.

References

Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, Batut P, Chaisson M, Gingeras TR. STAR: ultrafast universal RNA-seq aligner. Bioinformatics. 2013 Jan 1;29(1):15-21.

Batut P, Dobin A, Plessy C, Carninci P, Gingeras TR. High-fidelity promoter profiling reveals widespread alternative promoter usage and transposon-driven developmental gene expression. Genome Res. 2013 Jan;23(1):169-80.