Schema for TSRchitect RAMPAGE Embryos TSS - TSRchitect RAMPAGE Embryos Transcription Start Sites
  Database: dm6    Primary Table: tss_RAMPAGE_Dmel14_E14h Data last updated: 2021-04-19
Big Bed File: /gbdb/dm6/bbi/RAMPAGE_dedup/tsrchitect/Dmel14_E14h_tsrchitect.bb
Item Count: 8,811
Format description: RAMPAGE Peaks Identified by TSRchitect
fieldexampledescription
chromchr2LReference sequence chromosome or scaffold
chromStart15731775Start position in chromosome
chromEnd15731781End position in chromosome
nametsr_peaked_1223Name of RAMPAGE peak
score310Score from 0-1000
strand-+ or -
nTSSs4Count of TSSs
nTAGs223Count of tags
tsrPeak0.31Maximum tag count fraction for all TSS positions in the TSR
tsrWdth6Width of TSR
tsrTrq0.59TSR torque; measure of TSR balance
tsrSI0.03Shape index (SI) value of TSR
tsrMSI0.01Modified shape index (MSI) value of TSR

Sample Rows
 
chromchromStartchromEndnamescorestrandnTSSsnTAGstsrPeaktsrWdthtsrTrqtsrSItsrMSI
chr2L1573177515731781tsr_peaked_1223310-42230.3160.590.030.01
chr2L1574812215748123tsr_peaked_12241000-1711.0010.002.001.00
chr2L1575103515751036tsr_peaked_12251000-1351.0010.002.001.00
chr2L1575106715751075tsr_peaked_1226550-2550.558-0.321.010.01
chr2L1575229315752294tsr_peaked_5381000+1741.0010.002.001.00
chr2L1576274915762750tsr_peaked_12271000-1261.0010.002.001.00
chr2L1604999216049995tsr_peaked_539540+23070.5430.081.000.00
chr2L1616582916165830tsr_peaked_5401000+1491.0010.002.001.00
chr2L1616834216168343tsr_peaked_5411000+1611.0010.002.001.00
chr2L1625177216251773tsr_peaked_12281000-1351.0010.002.001.00

TSRchitect RAMPAGE Embryos TSS (tss_RAMPAGE_embryos) Track Description
 

Description

This track shows the locations of putative Transcription Start Sites (TSS) in the D. melanogaster genome based on the analysis of RAMPAGE data from different embryonic stages in one-hour intervals.

The embryos RAMPAGE datasets were retrieved from the Gene Expression Omnibus database at NCBI under the accession numbers GSE36212 and GSE89299. The RAMPAGE reads were mapped against the D. melanogaster (dm6) assembly using STAR. The putative promoter regions were identified by TSRchitect.

References

Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, Batut P, Chaisson M, Gingeras TR. STAR: ultrafast universal RNA-seq aligner. Bioinformatics. 2013 Jan 1;29(1):15-21.

Batut P, Dobin A, Plessy C, Carninci P, Gingeras TR. High-fidelity promoter profiling reveals widespread alternative promoter usage and transposon-driven developmental gene expression. Genome Res. 2013 Jan;23(1):169-80.