Description
This track was created by mapping D. mojavensis RNA-Seq reads against the
D. mojavensis DmojImproved genome assembly using
TrueSight.
The RNA-Seq data were generated by modENCODE project and the raw data is
available for download at the NCBI Sequence Read Archive (SRA):
Accession number | Description | Platform |
SRX054488 | SRR166836 |
Adult females (rep1) |
Illumina Genome Analyzer II |
SRX054489 | SRR166837 |
Adult females (rep2) |
Illumina HiSeq 2000 |
SRX247002 | SRR768439 |
Adult males (rep1) |
Illumina Genome Analyzer II |
SRX247003 | SRR768440 |
Adult males (rep2) |
Illumina HiSeq 2000 |
The TrueSight junction predictions from the different libraries are filtered
and merged together into a single set of predictions.
Methods
TrueSight was run against the D. mojavensis genome using the following
parameters: -i 30 -I 150000.
References
Li Y, Li-Byarlay H, Burns P, Borodovsky M, Robinson GE, Ma J.
TrueSight: a new
algorithm for splice junction detection using RNA-seq.
Nucleic Acids Res. 2013 Feb 1;41(4):e51
The RNA-Seq data were submitted by the modENCODE project.
The original RNA-Seq dataset can be obtained from the NCBI GEO database under the accession number
GSE28078.
|