RNA-Seq Coverage (Stages) Track Settings
 
RNA-Seq Coverage for Different Developmental Stages   (All RNA-Seq Tracks)

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Description

These tracks show the RNA-Seq read coverage for different developmental stages of D. kikkawai. The RNA-Seq reads were mapped against the D. kikkawai (DkikHiC1) genome assembly using HISAT2. RNA-Seq read alignments from multiple replicates were combined together, and the read coverage was normalized by a wigsum of 100,000,000 (1 million 100bp reads). The read coverage tracks were produced by the bam2wig.py program in RSeQC.

Accession Numbers for RNA-Seq Data

The RNA-Seq data were obtained from NCBI using the following accession numbers:

References

Chen ZX, et al. Comparative validation of the D. melanogaster modENCODE transcriptome annotation. Genome Res. 2014 Jul;24(7):1209-23.

Kim D, Langmead B, Salzberg SL. HISAT: a fast spliced aligner with low memory requirements. Nat Methods. 2015 Apr;12(4):357-60.

Wang L, Wang S, Li W. RSeQC: quality control of RNA-Seq experiments. Bioinformatics. 2012 Aug 15;28(16):2184-5.