Description
This track shows the exon junctions extracted from spliced RNA-Seq reads that
have been aligned to the genome. The splice junctions were identified by the
regtools junctions
extract subprogram.
The splice junction predictions from the different libraries are filtered
and merged together into a single set of predictions. The predictions are
color-coded based on the number of reads supporting the junction:
| Color | Number of reads |
|---|
| > 1000 |
| 500-999 |
| 100-499 |
| 50-99 |
| 10-49 |
| < 10 |
Accession Numbers for RNA-Seq Data
The RNA-Seq data were obtained from NCBI using the following accession numbers:
References
Chen ZX, et al. Comparative validation of
the D. melanogaster modENCODE transcriptome annotation. Genome Res. 2014
Jul;24(7):1209-23.
Kim D, Langmead B, Salzberg SL. HISAT: a
fast spliced aligner with low memory requirements. Nat Methods. 2015 Apr;12(4):357-60.
Wang L, Wang S, Li W. RSeQC: quality
control of RNA-Seq experiments. Bioinformatics. 2012 Aug 15;28(16):2184-5.
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