Track of fruitfly polytracts

Description

This tract displays polytracts in the fruitfly reference genome. A polytract is defined as a tract of tandem mono-nucleotide, di-nucleotide, or tri-nucleotide repeats, termed MNR, DNR, and TNR, respectively. Each MNR and DNR span at least six units, and each TNR spans at least three units. Incomplete terminal motif is included in a polytract (so that the length of a DNR or TNR is not necessarily multiple of 2 or 3).

Display Conventions and Configuration

Four shades of grayness are used to distinguish four classes of data, including three clades of polytracts (MNR, DNR, and TNR) and the polytract hinge.

•  - Mono-Nucleotide Repeat (MNR)
•   - Di-Nucleotide Repeat (DNR)
•   - Tri-Nucleotide Repeat (TNR)
•  - polytract break-point (hinge)

 Methods

A polytract is defined as a tract of mono-nucleotide, di-nucleotide, or tri-nucleotide tandemly repeated motifs, with possibly incomplete terminal motif included, where the minimum number of repeated units are 6 for MNR and 3 for DNR and TNR. Polytracts were identified through a string matching between a pattern and each chromosome sequence, with assistance from R packages ¡°stringr¡± (https://CRAN.R-project.org/package=stringr) and ¡°BSgenome.Dmelanogaster.UCSC.dm6¡± (www.bioconductor.org; DOI: 10.18129/B9.bioc.BSgenome). Accommodating the complementarity between a purine and pyrimidine pair (A:T and G:C), MNRs comprise A/T and G/C species, DNRs comprise TA, CT/GA, CA/GT, and GC species, and TNRs comprise AAC, AAG, AAT, ACC, GAC, ACT, CAG, AGG, ATC, and CGG species. That is, our resultant polytract dataset consisted of two types of MNRs, four types of DNRs, and ten types of TNRs.

Credits

Data were generated and processed in Guo Bioinformatics Lab at UNM Comprehensive Cancer Center. For inquiries, please contact Dr. Hui Yu (huiyu1@salud.unm.edu).

References

Yu H, Zhao S, Ness S, Kang H, Sheng Q, Samuels DC, Oyebamiji O, Guo Y. Non-canonical RNA-DNA differences and other human genomic features are associated with very short tandem repeats. PLoS Comp Biol. 2020 In revision.