D. melanogaster protein sequences from FlyBase were aligned against each
scaffold in the D. simulans (DsimGB2) assembly and the predicted gene models were constructed
D. melanogaster protein sequences were aligned against the D. simulans (DsimGB2) genome
assembly using NCBI TBLASTN with the following parameters:
The TBLASTN results were used by GeMoMa to produce an initial set of gene predictions.
The GeMoMa predictions are then filtered by the
GAF module in GeMoMa to
produce the final set of gene predictions.
Keilwagen J, Wenk M, Erickson JL, Schattat MH, Grau J, Hartung F.
Using intron position conservation for homology-based gene prediction.
Nucleic Acids Res. 2016 May 19;44(9):e89.