Description

These tracks show the read coverage (in reads per million) from Precision Run-On and sequencing (PRO-seq) of Drosophila S2 cells.

Adapter sequences were removed from the PRO-seq reads using cutadapt. The reverse complement of the trimmed reads were produced using the fastx_reverse_complement program in FASTX-Toolkit. These reads were mapped against the D. melanogaster genome using bwa, and only the reliably mapped reads were kept. The read coverage tracks were produced using the genomeCoverageBed program in bedtools.

Credits

The datasets were retrieved from the Gene Expression Omnibus database at NCBI under the accession number GSE77607.

Duarte FM, Fuda NJ, Mahat DB, Core LJ, Guertin MJ, Lis JT. Transcription factors GAF and HSF act at distinct regulatory steps to modulate stress-induced gene activation. Genes Dev. 2016 Aug 1;30(15):1731-46.