Schema for Combined MachiBase TSS - Combined MachiBase Transcription Start Sites
  Database: dm6    Primary Table: tss_MachiBase_Peaks    Row Count: 7,480   Data last updated: 2022-10-21
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 108131int(10) unsigned range
chromEnd 108142int(10) unsigned range
name 0_0varchar(255) values
score 0int(10) unsigned range
strand +char(1) values
thickStart 108131int(10) unsigned range
thickEnd 108142int(10) unsigned range
reserved 16711680int(10) unsigned range

This table points to a file in BigWig format.

Sample Rows
 
binchromchromStartchromEndnamescorestrandthickStartthickEndreserved
585chr2L1081311081420_00+10813110814216711680
585chr2L1129211130950_10+11292111309516766720
585chr2L1217461217510_00-12174612175116711680
585chr2L1232581234321_10-12325812343211177813
585chr2L1238811240293_10-123881124029255
585chr2L1262091262170_00-12620912621716711680
585chr2L1289661289870_00-12896612898716711680
586chr2L1320461321200_10+13204613212016766720
586chr2L1425251425942_00-1425251425945614080
586chr2L1553041556801_00+15530415568011162880

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

Combined MachiBase TSS (tss_MachiBase_Combined) Track Description
 

Description

These tracks show the normalized density of SAGE reads from different developmental stages of D. melanogaster. The datasets were produced by MachiBase and were obtained from the NCBI BioProject database under the accession number PRJNA79391.

Methods

Each dataset was mapped to the D. melanogaster release 6 assembly using bwa and the alignment results were analyzed by CAGEr. Consensus promoters across all the samples were produced by the consensusClusters function in CAGEr with the following parameters:

tpmThreshold = 5, qLow = 0.1, qUp = 0.9, maxDist = 100

Credits

Ahsan B, Saito TL, Hashimoto S, Muramatsu K, Tsuda M, Sasaki A, Matsushima K, Aigaki T, Morishita S. MachiBase: a Drosophila melanogaster 5'-end mRNA transcription database. Nucleic Acids Res. 2009 Jan;37(Database issue):D49-53.

Haberle V, Forrest AR, Hayashizaki Y, Carninci P, Lenhard B. CAGEr: precise TSS data retrieval and high-resolution promoterome mining for integrative analyses. Nucleic Acids Res. 2015 Apr 30;43(8):e51.