Schema for Combined Exo-seq TSS - Combined Transcription Start Sites Identified by Exo-seq
  Database: dm6    Primary Table: tss_exoseq_CAGE_Peaks    Row Count: 7,976   Data last updated: 2022-10-21
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 59236int(10) unsigned range
chromEnd 59264int(10) unsigned range
name 2_1varchar(255) values
score 0int(10) unsigned range
strand -char(1) values
thickStart 59236int(10) unsigned range
thickEnd 59264int(10) unsigned range
reserved 255int(10) unsigned range

This table points to a file in BigWig format.

Sample Rows
 
binchromchromStartchromEndnamescorestrandthickStartthickEndreserved
585chr2L59236592642_10-5923659264255
585chr2L72598726380_00+725987263816711680
585chr2L94734947492_10+9473494749255
585chr2L1023801024600_00+10238010246016711680
585chr2L1077481077970_00+10774810779716711680
585chr2L1081301081880_00+10813010818816711680
585chr2L1085891087650_00+10858910876516711680
585chr2L1129111131422_00+11291111314265280
585chr2L1204741204970_10-12047412049716766720
585chr2L1231781233612_10-123178123361255

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

Combined Exo-seq TSS (tss_exoseq_Combined) Track Description
 

Description

These tracks show the normalized density of the combined Exo-seq reads from D. melanogaster collected at different Zeitgeber Times (ZT) and temperatures. The Exo-seq datasets were obtained from the NCBI Sequence Read Archive database under the accession number SRP045351.

Methods

Each dataset was mapped to the D. melanogaster release 6 assembly using bwa with default parameters. The alignment results were analyzed by CAGEr. Consensus promoters across all the samples were produced by the consensusClusters function in CAGEr with the following parameters:

tpmThreshold = 5, qLow = 0.1, qUp = 0.9, maxDist = 100

Credits

Afik S, Bartok O, Artyomov MN, Shishkin AA, Kadri S, Hanan M, Zhu X, Garber M, Kadener S. Defining the 5′ and 3′ landscape of the Drosophila transcriptome with Exo-seq and RNaseH-seq. Nucleic Acids Res. 2017 Jun 20;45(11):e95.

Haberle V, Forrest AR, Hayashizaki Y, Carninci P, Lenhard B. CAGEr: precise TSS data retrieval and high-resolution promoterome mining for integrative analyses. Nucleic Acids Res. 2015 Apr 30;43(8):e51.