Schema for Combined modENCODE CAGE TSS - Combined Transcription Start Sites Identified by modENCODE CAGE
  Database: dm6    Primary Table: modECAGE_Combined_minus    Row Count: 1   Data last updated: 2022-10-21
fieldexampleSQL type info
fileName /gbdb/dm6/bbi/modENCODE_CAG...varchar(255) values

This table points to a file in BigWig format.

Sample Rows
 
fileName
/gbdb/dm6/bbi/modENCODE_CAGE/modENCODE_CAGE_Combined_minus.bw

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

Combined modENCODE CAGE TSS (tss_modECAGE_Combined) Track Description
 

Description

These tracks show the normalized density of CAGE reads from different developmental stages of D. melanogaster. The CAGE datasets were produced by the modENCODE project and were obtained from the NCBI Sequence Read Archive database under the accession number SRP001602.

Methods

Each dataset was mapped to the D. melanogaster release 6 assembly using bwa with default parameters. The alignment results were analyzed by CAGEr. Consensus promoters across all the samples were produced by the consensusClusters function in CAGEr with the following parameters:

tpmThreshold = 5, qLow = 0.1, qUp = 0.9, maxDist = 100

Credits

Hoskins RA, et al. Genome-wide analysis of promoter architecture in Drosophila melanogaster. Genome Res. 2011 Feb;21(2):182-92.

Haberle V, Forrest AR, Hayashizaki Y, Carninci P, Lenhard B. CAGEr: precise TSS data retrieval and high-resolution promoterome mining for integrative analyses. Nucleic Acids Res. 2015 Apr 30;43(8):e51.