Schema for Combined modENCODE CAGE TSS - Combined Transcription Start Sites Identified by modENCODE CAGE
  Database: dm6    Primary Table: tss_modECAGE_Peaks    Row Count: 14,110   Data last updated: 2022-10-21
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 7491int(10) unsigned range
chromEnd 7515int(10) unsigned range
name 0_0varchar(255) values
score 0int(10) unsigned range
strand +char(1) values
thickStart 7491int(10) unsigned range
thickEnd 7515int(10) unsigned range
reserved 16711680int(10) unsigned range

This table points to a file in BigWig format.

Sample Rows
 
binchromchromStartchromEndnamescorestrandthickStartthickEndreserved
585chr2L749175150_00+7491751516711680
585chr2L54856549340_00+548565493416711680
585chr2L55013550690_00+550135506916711680
585chr2L59230592691_20-592305926911177813
585chr2L67042670443_00+670426704465280
585chr2L71740717411_20+717407174111177813
585chr2L72597726173_20+7259772617255
585chr2L87376874002_00-87376874005614080
585chr2L94720947703_10+947209477032896
585chr2L1023811024363_10+10238110243632896

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

Combined modENCODE CAGE TSS (tss_modECAGE_Combined) Track Description
 

Description

These tracks show the normalized density of CAGE reads from different developmental stages of D. melanogaster. The CAGE datasets were produced by the modENCODE project and were obtained from the NCBI Sequence Read Archive database under the accession number SRP001602.

Methods

Each dataset was mapped to the D. melanogaster release 6 assembly using bwa with default parameters. The alignment results were analyzed by CAGEr. Consensus promoters across all the samples were produced by the consensusClusters function in CAGEr with the following parameters:

tpmThreshold = 5, qLow = 0.1, qUp = 0.9, maxDist = 100

Credits

Hoskins RA, et al. Genome-wide analysis of promoter architecture in Drosophila melanogaster. Genome Res. 2011 Feb;21(2):182-92.

Haberle V, Forrest AR, Hayashizaki Y, Carninci P, Lenhard B. CAGEr: precise TSS data retrieval and high-resolution promoterome mining for integrative analyses. Nucleic Acids Res. 2015 Apr 30;43(8):e51.