ChIP-Seq data for H3K4me2, H3K9me2, and H3K27me3 were analyzed using MUSIC
in order to identify regions in the D. melanogaster genome that are enriched for these histone
Reads from the two replicates were merged into a single file prior to the analysis with MUSIC.
Only the subset of scaffolds larger than 100kb in the D. melanogaster assembly were analyzed by MUSIC.
Broad peaks for H3K9me2 and H3K27me3 were identified using the -get_optimal_broad_ERs option. Punctated
peaks for H3K4me2 were identified using the -get_optimal_punctate_ERs option.
Harmanci A, Rozowsky J, Gerstein M.
MUSIC: identification of enriched regions in ChIP-Seq experiments using a mappability-corrected multiscale signal processing framework.
Genome Biol. 2014;15(10):474.